Solutions
What is a Solution?
A solution is defined as a solute (a smaller amount) dissolved in a solvent (larger amount). The concentration of a solution frequently must be known to a high degree of accuracy. An incorrectly prepared solution can destroy months of hard work or cost companies thousands of dollars. Therefore, companies usually have an SOP (Standard Operating Procedure) for the preparation of each solution in minimize mistakes. All calculations are recorded in the lab notebook, even if a calculator is used. Critical calculations are double-checked by another person (and sometimes triple-checked). The exact mass and volume of reagents used are recorded in the notebook. This information, along with the date and preparer's name or initials, is recorded on a solution preparation form and on a label on the bottle itself.
Calculating Dilutions
Preparing Solutions
Kool-Aid Chemistry Lab
We will be using Kool-aid to determine the concentration (molarity) of properly made Kool-Aid and make dilutions. We will be using the complete lab safety gear, lab coat, goggles and your hair will need to be tied back.
Solution Concentrations With Percent Solutions
Making Percent Solutions
You will be making different % solutions of Alcohol. You will then use these different percent concentrations of Alcohol to do DNA extraction of Strawberries.
1. Take the 99.5%
1. Take the 99.5%
Percent Solutions and DNA Extraction Lab
From a 100% Isopropyl Alcohol, you will make
25ml of
91%
70%
50%
Isoppropyl Alcohol
Show your calculations
Do a Strawberry DNA extraction
Find 2 journals to support why DNA extraction is important and includle both journals as a justification on DNA extraction
Answer the following
Why do strawberries make good DNA extraction models
What is important about the extraction solutions
We used soap and salt, why are they important. Other extractions use meat tenderizer or protease, why are they important.
What role do they have in the DNA extraction
25ml of
91%
70%
50%
Isoppropyl Alcohol
Show your calculations
Do a Strawberry DNA extraction
Find 2 journals to support why DNA extraction is important and includle both journals as a justification on DNA extraction
Answer the following
Why do strawberries make good DNA extraction models
What is important about the extraction solutions
We used soap and salt, why are they important. Other extractions use meat tenderizer or protease, why are they important.
What role do they have in the DNA extraction