Micropipetting and Gel Electrophoresis
Using tools in Biotechnology is very important. The tools we use are specific for the purpose of looking at microscopic levels and measuring in micro's. We will be looking at two tools that are used in biotechnology and why these are important.
Article-DNA Biological Functions and DNA in Biotechnology 2/4/19
You will read the following articles and you will write three brief summaries on each article. You will say who wrote it, where it was written and when it was written. You will summarize the articles by stating what they are about through breaking down each section of the article. This is an essay not a paragraph. So I expect multiple paragraphs. I also do not want your opinion on the articles. They should be at least 150 words each, but can be more.
After your summaries, I want an essay written about the articles you just read. How are they important to the study of Biotechnology and how do they help in explaining the role of DNA and the future or the current role of DNA. In this essay, you should write how each article is related. Talk about how they are tied to each other, how are they different. I would also like to hear your opinion on this topic.
I don't want to see that you enjoyed the article, that is not important.
THIS ESSAY WILL COUNT AS A TEST GRADE
These essay are due on Tuesday February 12 on Schoology.
After your summaries, I want an essay written about the articles you just read. How are they important to the study of Biotechnology and how do they help in explaining the role of DNA and the future or the current role of DNA. In this essay, you should write how each article is related. Talk about how they are tied to each other, how are they different. I would also like to hear your opinion on this topic.
I don't want to see that you enjoyed the article, that is not important.
THIS ESSAY WILL COUNT AS A TEST GRADE
These essay are due on Tuesday February 12 on Schoology.
Micropipetting Practice-2/7/19, 2/8/19
How to Use a Micropipet
These videos will help in how to use a micropipette. Watch these videos before we work on the pipets.
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We will practice using the micropittes by following the following worksheets.
Follow the second page of this worksheet. We will have some modifications
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Gel Electrophoresis
First thing you will do is watch the video on Gel Electrophoresis by the Ameoba Sisters. Then you will answer the questions from the handout that I will pass out in class. Because of copyright, I will not have that handout available to you on the website. This handout is due today. It should not take you the entire period to watch the video and answer the questions on your document. Make sure that you answer in proper complete sentences.
First thing you will do is watch the video on Gel Electrophoresis by the Ameoba Sisters. Then you will answer the questions from the handout that I will pass out in class. Because of copyright, I will not have that handout available to you on the website. This handout is due today. It should not take you the entire period to watch the video and answer the questions on your document. Make sure that you answer in proper complete sentences.
First thing you will do is watch the video on Gel Electrophoresis by the Ameoba Sisters. Then you will answer the questions from the handout that I will pass out in class. Because of copyright, I will not have that handout available to you on the website. This handout is due today. It should not take you the entire period to watch the video and answer the questions on your document. Make sure that you answer in proper complete sentences.
Watch the next videos on Pipetting Immersion and Gel Loading Practice
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Gel Loading Practice
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Flinn Scientific Inc
A Process to Dye For:Gel Electrophorsis
Electrophoresis Lab
Background Information
As it becomes more important for substances to be identified by their molecular structures rather than by direct observations, scientist are challenged with how to separate and isolate molecules from one another. Over the years, separation techniques were developed to separates and identify molecules. However, biological molecules like proteins and DNA are very large and sensitive and many of the separation techniques are not appropriate.
Gel electrophoresis is a process that involves applying an electrical current to a gelatin-like substance containing biological samples. When mixtures of materials are placed within the wells or openings of the gel and an electric current is applied, the molecules travel through the gel and separate from one another according to each molecule's charge, size, and shape. The gel, called arose, is made from an extract of red algae. It acts as a typo of molecular strainer or sieve and prevent all the molecules from moving too quickly. However, like pouring vegetable soup through a strainer, the smaller molecules typically move through gel at a faster rate that larger ones. When the current is turned off, all the molecules are stopped within the gel. If a dye is added to the samples placed in the wells, individual groups of molecules can be identified by a distinct, colored band within the gel. When the distance ah band (group of molecules) traveled is measured and compared to other colored bands within Hegel, the measurement can readily distinguish smaller, molecular weight molecules from larger molecules.
Gel electrophoresis is a process that involves applying an electrical current to a gelatin-like substance containing biological samples. When mixtures of materials are placed within the wells or openings of the gel and an electric current is applied, the molecules travel through the gel and separate from one another according to each molecule's charge, size, and shape. The gel, called arose, is made from an extract of red algae. It acts as a typo of molecular strainer or sieve and prevent all the molecules from moving too quickly. However, like pouring vegetable soup through a strainer, the smaller molecules typically move through gel at a faster rate that larger ones. When the current is turned off, all the molecules are stopped within the gel. If a dye is added to the samples placed in the wells, individual groups of molecules can be identified by a distinct, colored band within the gel. When the distance ah band (group of molecules) traveled is measured and compared to other colored bands within Hegel, the measurement can readily distinguish smaller, molecular weight molecules from larger molecules.
Experimental Overview
The purpose of this activity is to demonstrate the separation technique known as gel electrophoresis. This process will be used to identify dye samples by charge, molecular mass, and shape. The information will also be used to identify the composition of an "unknown " mixture of dyes.
You will first make your groups gel, then you will make the buffer solutions. When you are done do the pre-lab activity.
You will first make your groups gel, then you will make the buffer solutions. When you are done do the pre-lab activity.
Pre-Lab Activity
Go through the power point then answer the pre-lab Activity. This is due today 3/7/19(B day) or 3/8/19 (A day). You will upload this on Schoology.
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Flynn Scientific A Process to Dye For: Gel Electrophoresis Lab-3/11/19(B day), 3/12/19 (A day)
Watch the video as a guide to what you will be doing in the lab.
Post Lab Question
Answer the following questions in proper complete sentences. You will need to turn in both the handwritten paper and these questions to get full credit for this lab. This is due today 3/11/19 6th period, 3/12/19 1st period, 3/13/19 7th period.
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